The federation said it will demand that Canal Plus France withdraw the video and abstain from using the logo. The federation also intends to request compensation for its improper use.
It plans to seek the support of other federations in the suit.
The tennis federation received support from the International Tennis Federation, which viewed the joke about Nadal as an attack on its anti-doping program.
ITF president Francesco Ricci Bitti said in a statement that the TV channel should remove the video and "issue an apology to all Spanish tennis players, who have been unfairly implicated."
Gaucho wrote:First Nadal, now Federer. This is the first time since 2004 (36 tourneys) Federer did not reach at least the quarter finals in a Grand Slam.